Figure 2

Evidence for the presence of of ciguatoxins (CTXs) and gambieric acid D (GA D) in shark tissues. (a) Extracted ion chromatogram of I-CTX-1&2 [M+Na]⁺ at 1163.6125 and HRMS exact mass spectra of (b) I-CTX-1&2 [M+Na]⁺ at 1163.6125 and (c) I-CTX-5 (C₆₂H₉₀O₁₉) [M+Na]⁺ at 1161.6070, in fraction F12 from stomach; (d) extracted ion chromatogram of I-CTX-3&4 ([M+Na]⁺ at 1179.6084 and HRMS exact mass spectra of (e) I-CTX-3&4 [M+Na]⁺ at 1179.6084 and (f) I-CTX-6 (C₆₂H₉₀O₂₀) [M+Na]⁺ at 1177.5910, in stomach crude; (g) extracted ion chromatogram of GA D [M+NH₄]⁺ at 1216.7354 and (h) HRMS exact mass spectra of GA D [M+NH₄]⁺ at 1216.7354, in flesh crude extract, respectively.