Figure 1
Corticosterone alters surface GluN2B-NMDAR clustering. (A) Dendritic fragments of GluN2A- and GluN2B-SEP expressing neurons before and after exposure to corticosterone (100 nM). Scale bar = 5 µm, scale bar inset = 1 µm. (B) Example of fluorescence intensity of GluN2A- (n = 12 dendritic fields, N = 5 neurons) and GluN2B-SEP (n = 15 dendritic fields, N = 5 neurons) clusters over time. (C) Comparison of GluN2A- and GluN2B-SEP cluster fluorescence intensity before and after exposure to corticosterone. ***p < 0.001, paired Student t-test. (D) Live immunostaining of GluN2A and GluN2B subunits in Homer 1c-DsRed expressing neurons. Scale bar = 10 µm, scale bar inset = 5 µm. (E) Immunostaining of surface GluN2A and GluN2B subunits before and after exposure to corticosterone. Scale bar = 5 µm. (F) Comparison of the fluorescence intensity of GluN2A (n = 12 dendritic fields, N = 5 neurons) and GluN2B (n = 11 dendritic fields, N = 5 neurons) subunit clusters (expressed as ratio) -SEP before and after exposure to corticosterone (50 nM, 20 min). *p < 0.05, Student t-test.
