Figure 1
Effect of iCRT3 on Wnt/β-catenin activation and TNF-α production in LPS-stimulated macrophages. Cultured RAW 264.7 cells were pre-treated with iCRT3 at the indicated concentration for 50 min and then stimulated with LPS (1 ng/ml). (A) Before treatment, RAW264.7 macrophage cells were co-transfected with β-catenin/TCF response reporter TOP-TK-Luc or its control FOP-TK-Luc and an internal control pRL-TK. Luciferase activity was measured after 24 h LPS stimulation and expressed as the relative fold change compared to the untreated. (B) Supernatants from cultured RAW 264.7 cells were subjected to ELISA for measurement of TNF-α level after 4 h LPS stimulation. (C) Cell viability of RAW 264.7 cells was determined by MTS assay with viability of untreated cells considered to be 100%. (D) Western blotting against IκB and actin using total cell lysate after 15 min LPS stimulation. The image shown is representative of three independent experiments with bar graph from densitometric analysis of blots. Data were expressed as mean ± SEM obtained from two independent experiments (n = 4 per group). *P < 0.05 versus vehicle and # P < 0.05 versus LPS alone.
