Table 2 In vitro cytotoxic activities (IC50*, µM) on human cancer cell lines of free gemcitabine (dFdC) and its active phosphorylated forms (dFdCMP and dFdCTP) free or encapsulated in host 1.

From: Positively charged cyclodextrins as effective molecular transporters of active phosphorylated forms of gemcitabine into cancer cells

Compound

MCF7 MTT

MCF7 CellTiter-Blue®

CCRF-CEM

CCRF-CEM Ara-C/8C

dFdC

21 ± 0.8

16.1 ± 0.1

0.018 ± 0.007

7.4 ± 0.5

dFdCMP

29 ± 0.7

>35

0.033 ± 0.002

11.8 ± 0.7

dFdCTP

>36

>35

0.030 ± 0.003

10.9 ± 0.7

dFdCMP/host 1

3.5 ± 0.5

2.0 ± 0.1

0.010 ± 0.005

3.3 ± 0.2

dFdCTP/host 1

3.2 ± 0.6

2.7 ± 0.1

0.027 ± 0.002

1.2 ± 0.1

  1. *The value of IC50 was calculated from the dose–response curve presented as dependence of cell viability on the concentration of studied compounds.
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