Figure 5
From: Drug screening of cancer cell lines and human primary tumors using droplet microfluidics
One human nasopharyngeal cancer sample NAS1608 was shown as an example. (a) Merged images between brightfield and red fluorescence channel (Ex. 531/40 nm, Em. 593/40 nm) were taken before and after 10 μM Bortezomib treatment for 17 h. Droplets were maintained but their volume shrank due to water evaporation inside the humidified CO2 incubator. Scale bar indicates 25 μm. (b) Observed cell viability (y-axis) was plotted against different treatment conditions (x-axis) in human nasopharyngeal cancer sample NAS1608 before treatment. (c) Observed cell viability (y-axis) was plotted against different treatment conditions (x-axis) in human nasopharyngeal cancer sample NAS1608 after 17 h treatment. For both cell viability graphs, each dotted line indicated the mean cell viability of all droplets under the specified treatment conditions, whereas each dot indicated the mean cell viability of all cells in one droplet, and the size of the dot denoted the sample cell population size in one droplet. BZ denoted Bortezomib; Cis denoted Cisplatin.
