Figure 9 | Scientific Reports

Figure 9

From: Genotype-specific physiological and transcriptomic responses to drought stress in Setaria italica (an emerging model for Panicoideae grasses)

Figure 9

Regulation of reactive oxygen species (ROS) in ‘Yugu1’ and ‘An04’ genotypes. (A) Expression profiles of featured genes involved in ROS scavenging in ‘An04’ and ‘Yugu1’. (B and C) The histochemical staining analysis shows that the ROS accumulation (indicated by 3,3′-diaminobenzidine staining,) and cell death (indicated by Trypan blue staining) triggered by drought were more severe in drought-sensitive ‘An04’ than in ‘Yugu1’. (D) The content of H2O2 in the leaves extracts of ‘Yugu1’ and ‘An04’. Values are means ± SE of three independent experiments. (E,F) 3,3′-diaminobenzidine and Trypan blue stains were quantified with mean ± SE (three biological replications) using ImageJ software (https://imagej.net/). P values were calculated using Welch’s two-sample t-test (*P < 0.05, **P < 0.001).

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