Figure 1 | Scientific Reports

Figure 1

From: PHF20 positively regulates osteoblast differentiation via increasing the expression and activation of Runx2 with enrichment of H3K4me3

Figure 1

Expression profiles of PHF20 during osteoblast differentiation. Osteoblast lineage MC3T3-E1 and C3H10T1/2 cells were differentiated in osteogenic medium (OM) for 0, 2, 4, and 8 days. Expression levels of PHF20 and osteoblast differentiation markers were determined by RT-PCR (A,C), qRT-PCR (B), and Western blot (D). β-actin was used as internal control. Arrows (→) (A,C,D) indicate cropping lines and gels/blots were obtained under the same experimental conditions. Values are expressed as means ± SEM (n = 3). *p < 0.05, **p < 0.01 and ***p < 0.001 compared to Day 0.

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