Figure 2 | Scientific Reports

Figure 2

From: PHF20 positively regulates osteoblast differentiation via increasing the expression and activation of Runx2 with enrichment of H3K4me3

Figure 2

Overexpression of PHF20 promotes osteoblast differentiation. MC3T3-E1 cell were infected with Ad-PHF20 (+, 50 MOI; ++, 100 MOI) or Ad-GFP (+, 50 MOI) as Mock and cultured for 24 hours with OM for qRT-PCR (A). Western blot analysis (B). At 24 hours after adenovirus infection, cells were cultured with OM for ALP staining (C) and alizarin red staining (AR-S) (D). Arrows (→) indicate cropping lines and blots were obtained under the same experimental conditions (B). ALP staining was performed with BCIP/NBT solution at 3 days. AR-S was performed at 8 days. For quantitation, ALP stained cells were quantified using ImageJ software (C, lower). Alizarin red stained cells were extracted with 10% (w/v) cetylpyridinium chloride and the absorbance value at 562 nm was then measured by spectrophotometry (D, lower). Values are expressed as means ± SEM (n = 3). *p < 0.05, **p < 0.01 and ***p < 0.001 compared to Ad-Mock.

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