Figure 1
From: Levobuipivacaine-Induced Dissemination of A549 Lung Cancer Cells
Effects of ropivacaine, lidocaine, bupivacaine and levobupivacaine on cell barrier function and EMT of A549 lung cancer cells. (A) Barrier function of lung cancer cells with and without local anaesthetics treatment as assessed by electric cell-substrate impedance sensing (ECIS). Measurements were performed at 4 kHz. (B) Effect of levobupivacaine on the morphology of A549 cells. Levobupivacaine treatment induced morphological change after 24 hr exposure. (C) Immunofluorescence staining of E-cadherin (E-cad), N-cadherin (N-cad) and vimentin for A549 cells treated with or without levobupivacaine. Ropi indicates ropivacaine; Lido indicates lidocaine; Bupi indicates bupivacaine, Levo indicates levobupivacaine; and Ctrl indicates control. Scale bar = 200 μm.
