Figure 3 | Scientific Reports

Figure 3

From: iNOS promotes hypothalamic insulin resistance associated with deregulation of energy balance and obesity in rodents

Figure 3

Exercise reverses insulin S-nitrosation in hypothalamus and normalizes energy homeostasis. Immunoblotting analysis of iNOS expression generated from lean and DIO rats in distinct hypothalamic nuclei (A) The biotin switch method was used to determine IRβ and Akt S-nitrosation in arcuate, ventromedial (VMH) and paraventricular (PV) nuclei of lean and DIO rats (B) (n = 10 animals per group). Effects of exercise on food intake (C). Immunoblotting analysis of iNOS, eNOS and nNOS expression generated from lean and diet induced obesity (DIO) rats submitted to physical exercise (D). The biotin switch method was used to determine IRβ and Akt S-nitrosation in hypothalamus of exercised lean and DIO rats (E). Western Blotting method was used to determine insulin-induced phosphorylation of IRβ and Akt (F). Data are presented as mean ± SEM, *p < 0.05 vs. lean groups (n = 8–10 animals per group).

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