Figure 4 | Scientific Reports

Figure 4

From: iNOS promotes hypothalamic insulin resistance associated with deregulation of energy balance and obesity in rodents

Figure 4

Pharmacological and genetic inhibition of iNOS reverses S-nitrosation in hypothalamus and normalizes energy homeostasis. Effects of L-NIL on food intake in hypothalamus of L-NIL treated lean and DIO rats (A). The biotin switch method was used to determine IRβ and Akt S-nitrosation in hypothalamus DIO and DIO_L-NIL (B). Western Blotting method was used to determine insulin-induced phosphorylation of IRβ and Akt in rats treated with vehicle or L-NIL in lean and DIO rats treated with L-NIL (C). Effects of iNOS antisense oligonucleotide (ASO) (D) on food intake (E), body weight change (F) in lean and DIO mice. Data are presented as mean ± SEM, *p < 0.05 vs. vehicle-injected rats; # p < 0.05 vs. respective lean group (n = 6–8 animals per group).

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