Figure 5
From: Multiple E3s promote the degradation of histone H3 variant Cse4
Cse4 overexpression leads to toxicity and cell cycle delay in psh1Δ but not ubr1Δ mutant cells. (A) Cse4 overexpression causes slower growth in cells lacking PSH1 or RCY1. A plasmid expressing GAL1 promoter-regulated Cse4 was transformed into wild-type, ubc4Δ, psh1Δ, ubr1Δ, rcy1Δ, slx5Δ cells as indicated. Yeast cells were grown to similar densities and 10-fold serial dilutions were spotted onto both glucose (SD, expression off) and galactose (SG, expression on) plates. The identities of these mutants are listed to the left of the panels. (B) Effects of Cse4 overexpression on cell cycle progression monitored by FACS. Cells were arrested in G1 triggered by α factor for 4 h. Cse4 expression was induced for 2 h in G1 arrested cells. The cells were released from G1 arrest and maintained in galactose-containing media. The samples collected were subject to FACScan analysis of DNA content. Representative FACS results are shown.
