Figure 7
From: Multiple E3s promote the degradation of histone H3 variant Cse4
Impaired Cse4 degradation and ubiquitylation in the psh1Δ rcy1Δ slx5Δ ubr1Δ quadruple mutant cells. (A,B) Cse4 degradation in wild-type and psh1Δ rcy1Δ slx5Δ ubr1Δ cells was determined and quantified as above. (C) Cse4 ubiquitylation was determined as described in Fig. 3C. (D,E) Degradation of Pex29 and Dbf4 in wild-type and psh1Δ rcy1Δ slx5Δ ubr1Δ cells. Pex29 and Dbf4 are regulated by the E3s Doa10 and APC, respectively31. The degradation kinetics of GST-tagged Pex29 and MORF-tagged Dbf4 were determined as previously described31. (F) Phenotypes associated with the quadruple mutant psh1Δ rcy1Δ slx5Δ ubr1Δ. Mutants display stress-sensitive phenotypes. Yeast wild-type cells and mutants lacking one or four E3s were spotted in 10-fold serial dilution onto YPD plates with the indicated agents and concentrations.
