Figure 3

Suppression of hepatic GRK2 restores glucose homeostasis and insulin sensitivity in ob/ob mice. (A–D) Plasma levels of glucose (A,B) and insulin (C,D) were determined during a IGTT (glucose 2.0 g/kg intraperitoneally [i.p.]) performed 24 h after GRK2 siRNA injection. The AUC of glucose (B) and insulin (D). (E,F) Plasma levels of glucose (E) and insulin (F) were determined during an IITT (insulin 0.75 U/kg i.p.) performed after 24 h of GRK2 siRNA injection. Values are mean ± SE; n = 6. ^* p < 0.05, ^** p < 0.01, ^*** p < 0.001 vs. control siRNA-transfected lean mice (lean-cont si); ^†† p < 0.01, ^††† p < 0.001 vs. control siRNA-transfected ob/ob mice (ob/ob-cont si) by repeated measures two-factor ANOVA test followed by Bonferroni correction for multiple comparisons (A,C,E,F) or one-way analysis of variance (B,D).