Figure 7
From: Suppression of GRK2 expression reduces endothelial dysfunction by restoring glucose homeostasis
Normalization of glucose concentration improved impaired insulin signaling in HUVECs induced GRK2 under HG/HI conditions. HUVECs were incubated for 72 h in HG (22 × 10−3 mol/L)/HI (10−7 mol/L) medium, and then the medium was replaced with LG (5 × 10−3 mol/L) for 0-24 h. Finally, insulin (10−6 mol/L) was added for 20 min. GRK2 (A), phospho-Akt (C) and eNOS (D) were tested by western blotting assay. (B) Levels of GRK2 activity in HUVECs. Protein levels of phosphorylation were normalized to the levels of total protein. Basically, Insulin-stimulated and non-stimulated lanes were run on the same gel but were noncontiguous, and partly, were run on a separated gel but under the same conditions. Values are mean ± SE; n = 6. †p < 0.05, ††p < 0.01, †††p < 0.001 vs. insulin-stimulated HUVECs incubated for 72 h in HG/HI medium and just behind the medium was replaced with LG (insulin-stimulated 0 h) by one-way analysis of variance.
