Table 5 Primers for the analysis of mRNAs for the validation of microarray data.
Gene | Forward | Reverse | |
|---|---|---|---|
1. | F3 | 5′-aggcactacaaatactgtggca-3′ | 5′-gcttcacatccttcacaatctcg-3′ |
2. | BMP2 | 5′-gcagcttccaccatgaagaatc-3′ | 5′-aaagcatcttgcatctgttctcg-3′ |
3. | TGFBR1 | 5′-tcacagagaccacagacaaagtt-3′ | 5′-aaagggccagtagttggaagtt-3′ |
4. | EDNRB | 5′-catgcgaaacggtcccaatatc-3′ | 5′-gactcagcacagtgattccca-3′ |
5. | ALOX12 | 5′-atggtcatccagattcagcctc-3′ | 5′-aggtgagtgttcagcaagtgata-3′ |
6. | NOX4 | 5′-caccctgttggatgactggaa-3′ | 5′-actgaggtacagctggatgttg-3′ |
7. | SELE | 5′-tcaagtgtgagcaaattgtgaac-3′ | 5′-attctccagaggacatacactgc-3′ |
8. | VCAM1 | 5′-tgaccttcatccctaccattga-3′ | 5′-gcatgtcatattcacagaactgc-3′ |
9. | ICAM1 | 5′-acagtcacctatggcaacgac-3′ | 5′-gtcactgtctgcagtgtctcct-3′ |
10. | ICAM2 | 5′-acagccacattcaacagcac-3′ | 5′-agatgtcacgaacagggacag-3′ |
11. | IL6 | 5′-ctgcaggacatgacaactcatc-3′ | 5′-atctgaggtgcccatgctac-3′ |
12. | CXCL8 | 5′-tcctgatttctgcagctctgt-3′ | 5′-tgtggtccactctcaatcactc-3′ |
13. | IL1A | 5′-gcttcctgagcaatgtgaaatac-3′ | 5′-tgacttataagcacccatgtcaa-3′ |
14. | IL1RN | 5′-gatacttgcaaggaccaaatgtc-3′ | 5′-gtctcatcaccagacttgacaca-3′ |
15. | CCL2 | 5′-caccaataggaagatctcagtgc-3′ | 5′-tgagtgttcaagtcttcggagtt-3′ |
16. | EDN1 | 5′-gagaaacccactcccagtcc-3′ | 5′-gatgtccaggtggcagaagt-3′ |
17. | SERPINE1 | 5′-tcaagcaagtggactttt-3′ | 5′-gttgaagtagagggcatt-3′ |
18. | PLAT | 5′-gaaccacaactactgcagaaacc-3′ | 5′-gtgctgtgtaaaccttgcctatc-3′ |
19. | ACTB | 5′-ggcatcctcaccctgaagta-3′ | 5′-ggggtgttgaaggtctcaaa-3′ |
