Figure 1
From: Silencing of TGF-β1 in tumor cells impacts MMP-9 in tumor microenvironment
In vivo analysis of tumor versus stromal gene expression at various stages of tumor progression. MDA-MB-435 cells were subcutaneously injected into 8 week-old athymic nude mice. Primary tumors and lung metastases were allowed to develop over a 12 week period. From cohorts of mice, both primary tumor material and metastatic lesions in the lung were collected and RNA was isolated for analysis of TGF-β1, MMP2 and MMP9 expression in the tumor and stromal compartments. Species-specific primers were used to analyze gene expression in different compartments. Analysis of tumor (human) gene expression at the primary site on week-3 and on week-6 (A) and in the lung metastasis (B), as determined by QRT-PCR is shown. Those specific for stromal gene (mouse) expression at the primary site and in lung metastasis are shown in (C) and (D), respectively. *p < 0.05; ***p < 0.001.
