Figure 3
From: Silencing of TGF-β1 in tumor cells impacts MMP-9 in tumor microenvironment
Co-culture assay to determine effects of paracrine signaling from tumor cells and CAFs. MDA-MB-435 (A) and MDA-MB-435TGFβsi (B) cells were cultured in CAF conditioned-medium for 72 hrs and then analyzed for proliferation using MTT assay. Similarly, CAFs were also cultured in conditioned medium from either MDA-MB-435 (C) or MDA-MB-435TGFβsi (D) cells for 72 hrs and changes were determined by MTT assay. *p < 0.05.
