Figure 4
From: Silencing of TGF-β1 in tumor cells impacts MMP-9 in tumor microenvironment
Co-culture assays to determine the effects of paracrine signaling on tumor and stromal cell gene expression. MDA-MB-435 cells and MDA-MB-435 TGF-βsi cells were co-cultured with fibroblasts in Boyden chambers. Forty-eight hrs later, RNA was collected from both the tumor and fibroblasts for RT-PCR analysis of TGF-β1, TGF-β2, TGF-β3, TGFβRI, TGFβRII, MMP-2 and MMP-9 gene expression levels. Values provided in (A) indicate gene expression from MDA-MB-435 cells, grown with CAFs; (B) in CAFs, grown with MDA-MB-435 cells; (C) in CAFs, grown with MDA-MB-435TGFβsi cells. *p < 0.05; **p < 0.01; ***p < 0.001.
