Table 1 Primers used in quantitiative real-time PCR.
From: Silencing of TGF-β1 in tumor cells impacts MMP-9 in tumor microenvironment
Gene | Human | Mouse |
|---|---|---|
TGF-β1 | F.P. 5′-GAGGGGAAATTGAGGGCTTT-3′ | F.P. 5′-CACCGGAGAGCCCTGGATA-3′ |
R.P. 5′-CGGTAGTGAACCCGTTGATG-3′ | R.P. 5′-TGTACAGCTGCCGCACACA-3′ | |
MMP-2 | F.P. 5′-GAAGGTGAAGGTCGGAGT-3′ | F.P. 5′-CACACCAGGTGAACCATGTG-3′ |
R.P. 5′-GAAGATGGTGATGGGATTTC-3′ | R.P. 5′-AGGGCTGCATTGCAAATATC-3′ | |
MMP-9 | F.P. 5′-TGGGCTACGTGACCTATGACAT 3′ | F.P. 5′-GTATGGTCGTGGCTCTAAGC-3′ |
R.P. 5′-GCCCAGCCACCTCCACTCCTC-3′ | R.P. 5′-AAAACCCTCTTGGTCTGGGG-3′ | |
GAPDH | F.P. 5′-ATTGCCCTCAACGACCACTT-3′ | F.P. 5′-ACCACAGTCCATGCCATAC-3′ |
R.P. 5′-TTACTCCTTGGAGGCCATGT-3′ | R.P. 5′-CACCACCCTTGTTGCTGTAGCC-3′ | |
TGF-βR1 | F.P. 5′-TGGGACCCACTTCCATTTCCTTCA-3′ | |
R.P. 5′-TCCCAAGCCTCATCTGCTCAATCT-3′ | ||
TGF-βR2 | F.P. 5′-TGTTGAGTC CTTCAAGCAGACCGA-3′ | |
R.P. 5′-ACTTCTCCCACTGCATTACAGCGA-3′ | ||
bFGF | F.P. 5′-CTTTGGCTGCTACTTGGAGG-3′ | |
R.P. 5′-GAAGCTTTCCAGCAAAGTGG-3′ | ||
VEGF | F.P. 5′-AGGAGGGCAGAATCATCACG-3′ | |
R.P. 5′-CAAGGCCACGGGGATTTTCT-3′ | ||
SDF-1 | F.P. 5′-ATGAACGCCAAGGTCGTGGTC-3′ | |
R.P. 5′-CTTGTTTAAAGCTTTCTCAGGTACT-3′ |
