Figure 1

Schematic of ROX SE perfusion in mice. In the ROX SE perfusion technique, the following solutions are perfused in sequence: 1) 10 mL of 10 mM glucose-PBS-Heparin, 2) 20 mL of 10 mM glucose-PBS, 3) 20 mL of 20 μM ROX SE solution, and 4) 20 mL of 4% paraformaldehyde. Perfusion pressures are maintained at 80 ± 5 mm Hg as indicated by the attached sphygmomanometer.