Figure 3
van’t Hoff plots for the interaction of FIIRE-RNA with IRP1 repressor protein in the absence and presence of Mn2+. The data are indicated as FRT IRE-RNA (‒○‒), FRT IRE-RNA + 50 µM Mn2+ (‒⦁‒), ACO2 IRE-RNA (‒∆‒) and ACO2 IRE-RNA + 50 µM Mn2+ (‒▲‒) with IRP1 protein. Enthalpy and Entropy were determined from the slope and intercept of the temperature dependent equilibrium binding measurements, respectively.
