Table 2 Thermodynamic parameters of enthalpy (ΔH), entropy (ΔS) and free energy change (ΔG) for the interactions of FRT and ACO2 IRE-RNA with IRP1 protein in the absence and presence of 50 µM Mn2+.

From: Thermodynamic and Kinetic Analyses of Iron Response Element (IRE)-mRNA Binding to Iron Regulatory Protein, IRP1

Complex

ΔH (kJ/mol)

ΔS (J/mol/K)

ΔG (kJ/mol)

FRT IRE-RNA/IRP1

−38.4 ± 2.4

21.1 ± 1.3

−44.0 ± 2.8

FRT IRE-RNA-Mn2+/IRP1

−23.6 ± 1.3

51.0 ± 3.3

−38.0 ± 2.1

ACO2 IRE-RNA/IRP1

−27.9 ± 1.4

38.5 ± 2.7

−39.3 ± 1.9

ACO2 IRE-RNA-Mn2+/IRP1

−11.2 ± 0.6

79.7 ± 4.8

−34.0 ± 2.2

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