Table 3 Temperature dependent kinetic rate constants for the interaction of FRT and ACO2 IRE-RNA with IRP1 in the absence and presence of 50 µM Mn2+.

From: Thermodynamic and Kinetic Analyses of Iron Response Element (IRE)-mRNA Binding to Iron Regulatory Protein, IRP1

Complex

Observed Rate Constant, k 2 (s−1)

Ea (kJ/mol)

5 °C

10 °C

15 °C

20 °C

25 °C

FRT IRE-RNA/IRP1

98 ± 4.4

178 ± 5.7

263 ± 6.5

319 ± 7.6

397 ± 8.5

47.0 ± 2.5

FRT IRE-RNA-Mn2+/IRP1

33 ± 2.1

46 ± 2.8

57 ± 3.0

69 ± 2.9

78 ± 3.6

29.0 ± 1.6

ACO2 IRE-RNA/IRP1

20 ± 1.8

26 ± 1.6

38 ± 2.0

45 ± 2.1

53 ± 2.4

35.0 ± 2.0

ACO2 IRE-RNA-Mn2+/IRP1

11 ± 0.7

14 ± 0.6

17 ± 1.4

20 ± 0.9

23 ± 1.7

25.0 ± 1.2

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