Figure 2

Label-free quantitative characterization of lung- and bone marrow-derived MSC proteome profiles using DIA-MS. (A) Venn diagram of the total number of proteins identified by quantitative proteomics in each sample group (BM-MSC conditioned medium (n = 3), BM-MSC cell layer (n = 3), lung-MSC conditioned medium (n = 3) and lung-MSC cell layer (n = 3)). Volcano plots for cell layer (B) and conditioned medium (C) for lung-MSC vs. bone marrow-MSC. Green dots indicate proteins that are significantly differentially expressed and have a fold change of ≥4 or ≤−4 and a p-value ≤ 0.05. The p-values were Benjamini and Hochberg corrected for multiple testing and presented as −log10. BM, bone marrow-derived MSC; Lung, lung-derived MSC; CL, cell layer; CM, conditioned medium.