Figure 5

Label-free quantitative characterization of lung- and bone marrow-derived MSC matrisome (ECM) profiles using DIA-MS. (A) Venn diagram of the total number of extracellular matrix (matrisome) proteins identified by quantitative proteomics in each sample group (BM-MSC conditioned medium (n = 3), BM-MSC cell layer (n = 3), lung-MSC conditioned medium (n = 3) and lung-MSC cell layer (n = 3)). (B) A table of matrisome proteins only identified in one group of four. (C) A heat map of the significantly differentially expressed matrisome proteins with a fold change of ≥4 or ≤−4 found in the cell layer of BM-MSC (n = 3) and Lung-MSC (n = 3). Unsupervised hierarchical clustering reveals clear differences in matrisome profile between BM-MSC and lung-MSC. Data are presented as z-score, where green color codes for higher expression and red color codes for lower expression. BM, bone marrow-derived MSC; Lung, lung-derived MSC; CL, cell layer; CM, conditioned medium.