Figure 6

Verification of CYP1B1 by SRM-MS and functional evaluation of its effect on proliferation. (A) Selected reaction monitoring analysis of CYP1B1 expression before and after stimulating lung-derived MSC with 10 µM Benzo[a]pyrene (BaP) for 24 hours (n = 4). (B) Stimulating Lung-MSC with 10 µM BaP for 24 hours decreased the proliferation rate (n = 5). No significantly increase in cell death was observed when stimulating Lung-MSC with 10 µM BaP up to 48 hours according to LDH analysis (n = 4) (C) or Trypan blue staining’s (n = 2) (D). Data are presented as mean (±SD).