Table 3 Comparisons of Rf value of the phytotoxins isolated from Alternaria species with standards of tenuazonic acid, alternariol, alternariol monomethyl ether by TLC

From: Isolation, characterization and toxicological potential of Alternaria-mycotoxins (TeA, AOH and AME) in different Alternaria species from various regions of India

Rf values

Chloroform: methanol (80:20)

Benzene: acetone: acetic acid (60:35:5)

Chloroform: methanol (95:5)

Ethyl acetate: benzene (95:5)

Standard TeA

0.63 ± 0.02

0.56 ± 0.03

0.42 ± 0.04

0.23 ± 0.05

Isolated toxins

0.62 ± 0.03

0.55 ± 0.01

0.41 ± 0.07

0.22 ± 0.06

Standard AOH

0.66 ± 0.09

0.59 ± 0.04

0.49 ± 0.03

0.31 ± 0.01

Isolated toxins

0.67 ± 0.04

0.60 ± 0.02

0.48 ± 0.04

0.32 ± 0.02

Standard AME

0.82 ± 0.01

0.78 ± 0.05

0.61 ± 0.09

0.39 ± 0.04

Isolated toxins

0.81 ± 0.06

0.77 ± 0.04

0.60 ± 0.02

0.38 ± 0.05

  1. Note: Rf value is the ratio of the distance travelled by the substance and the distance travelled by the solvent (The spots were developed by spraying the plates with 0.2% FeCl3 in ethanol and some are detected in UV-light at 365 nm).
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