Table 2 Ranking of candidate reference genes according to stability values in a pool of Urochloa brizantha leaves samples subjected to various abiotic stresses.

From: Identification of Endogenous Reference Genes for RT-qPCR Expression Analysis in Urochloa brizantha Under Abiotic Stresses

Gene Name

geNorm

NormFinder

BestKeeper

ΔCt

RefFinder

Rank order

Average expression stability (M)

Rank order

Stability Value (SV)

Rank order

Standard deviation [ + /−CP]

Rank order

Average of standard deviations

Rank order

Geometric mean of ranking values

Heat Stress

EF1-α

6

1,297

1

0,727

5

1,21

1

1,35

1

2,34

U2AF

2

0,813

4

0,988

2

0,78

4

1,44

2

2,38

β-TUB6

1

0,813

8

1,472

1

0,76

8

1,73

3

2,83

Act12

5

1,231

2

0,788

6

1,46

2

1,39

4

3,31

α-TUB5

3

0,976

5

1,009

3

1,12

5

1,47

5

3,87

eIF4a

7

1,374

3

0,943

8

2,02

3

1,41

6

4,74

SucCoa

4

1,109

6

1,018

4

1,19

6

1,49

7

4,90

FTSH

8

1,453

7

1,360

9

2,35

7

1,68

8

7,71

UbiCo

9

1,529

9

1,517

7

1,77

9

1,80

9

8,45

Cold Stress

EF1-α

1

0,667

4

0,592

1

0,84

3

1,18

1

1,86

UbiCo

3

0,824

1

0,501

4

0,91

1

1,15

2

1,86

α-TUB5

2

0,667

2

0,549

5

0,90

2

1,16

3

2,11

eIF4a

4

0,915

3

0,591

6

0,98

4

1,18

4

4,12

SucCoa

5

1,010

5

0,992

8

1,24

5

1,40

5

5,62

Act12

7

1,109

8

1,360

2

0,87

8

1,55

6

5,66

FTSH

8

1,206

7

1,247

3

0,90

7

1,55

7

5,66

β-TUB6

6

1,105

6

1,237

9

1,52

6

1,54

8

6,64

U2AF

9

1,392

9

1,496

7

1,00

9

1,73

9

8,45

Drought Stress

eIF4a

1

0,565

1

0,656

6

0,96

1

1,26

1

1,57

U2AF

2

0,565

2

0,761

3

0,92

2

1,31

2

1,86

α-TUB5

5

1,208

5

1,155

1

0,74

6

1,54

3

3,50

SucCoa

6

1,261

3

0,772

4

0,93

3

1,33

4

3,83

β-TUB6

4

1,110

6

1,210

2

0,78

5

1,53

5

3,94

EF1-α

3

1,023

8

1,301

5

0,95

7

1,61

6

5,38

Act12

7

1,324

4

0,867

7

1,03

4

1,36

7

5,29

FTSH

8

1,414

7

1,294

8

1,11

8

1,64

8

7,74

UbiCo

9

1,474

9

1,437

9

1,51

9

1,68

9

9,00

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