Figure 1
Analysis of gene expression profiles between Huh7.5 and Huh7.5M cells. (a) Major pathways regulated during EMT in Huh7.5:Huh7.5M system. Gene expression changes were analyzed by IPA. Red shades represent the genes upregulated in Huh7.5M cells while the green shades represent those downregulated. Numbers at the top of each histogram represents the total number of genes classified in the respective category. Left Y-axis represents the percentage of genes in the respective category that underwent regulation. Right Y axis corresponds to the –log of the P- value generated by right tailed Fisher’s exact test. (b) Comparison of EMT core gene expression between in Huh7.5:Huh7.5M system and that generated by Groger et al. Gene expression profiles of Huh7.5 and Huh7.5M cells were used for this comparison. “Agree” corresponds to the genes that underwent changes as suggested by the reference list; “Disagree” has those genes that were regulated in disagreement with the reference list; and “Unaltered” suggests that these genes were not detected, unlike in the reference list. (c) Validation of microarray results of eleven genes from EMT core list by qRT-PCR. Fold change in Huh7.5M cells over Huh7.5 cells from microarray and qRT-PCR experiments are represented. *, ** and *** indicate statistical significance of qRT-PCR data represented as P-values < 0.05, < 0.005 and < 0.0005 respectively.
