Figure 2
Validation of microarray results. (a) Huh7.5 and Huh7.5M cells were cultured up to 70% confluency, harvested and lysates prepared. Equal quantities of lysates were electrophoresed and subjected to immunobloting against the antigen mentioned. (b) Quantitative representation of results from (a). Intensities of the bands were analyzed by densitometry, normalized to those of the corresponding β-Tubulin or GAPDH and plotted graphically. (c) Comparison of FAK phosphorylation between the two cells. The intensities of the corresponding bands were normalized against that of the total protein and further normalized against those of GAPDH. Relative expressions of representative genes from TCA and OXPHOS (d), glycogen biosynthesis (e), cholesterol and bile acid biosynthesis (f) and fatty acid biosynthesis and degradation (g) pathways in Huh7.5 and Huh7.5M cells, quantified by qRT-PCR as described earlier. (h) demonstrates relative expression levels of representative liver-specific genes in Huh7.5 and Huh7.5M cells, quantified by qRT-PCR. *, ** and *** indicate P-values < 0.05, < 0.005 and < 0.0005 respectively.
