Figure 5
Analysis of GSK-3β signaling in Huh7.5M cells. (a) Huh7.5M cells were treated with 10 µM CHIR99021 for 1 hr at 70% confluency. Cells were harvested post-treatment and subjected to immunobloting. (b) and (c) describe quantitative representation of relative expressions of E-Cadherin and Vimentin in Huh7.5M cells from (a). (d) and (e) depict relative transcript levels of CDH1 and VIM in Huh7.5M cells upon inhibition by CHIR99021 quantified by qRT-PCR. (f) Relative TOP-luciferase activity measured from Huh7.5M cells treated with CHIR99021. Dual luciferase assay was performed 30 hrs post-transfection of cells with TOP-Fluc and pCMV-Rluc plasmids. One hour prior to the harvest, cells were treated with the inhibitor. (g) GSK-3β inhibition and analysis of EMT markers in MDA-MB-231 cells similar to (a). 50 µM CHIR99021 was used in inhibition. * indicates P-value < 0.05. NS-non significant.
