Figure 6
Regulation of GSK-3β signaling by p38MAPK and ERK1/2 in Huh7.5M cells. (a) Huh7.5M cells treated with 50 µM of p38MAPK inhibitor VIII or 100 µM of U0126 or both of them for 1 hr were lysed and the effects were analyzed by immunobloting. (b) Effect of GSK-3β inhibition on p38MAPK and ERK1/2 in Huh7.5M cells treated with 10 µM CHIR99021 for 1 hr analyzed by immunobloting as in (a). (c) Consequence of p38MAPK and ERK1/2 inhibitions on GSK-3β signaling in MDA-MB-231 cells. Cells were treated with 50 µM of p38MAPK inhibitor VIII or 50 µM of U0126 individually or together for 1 hr before harvesting. (d) Effect of wt and kd-GSK-3β expressions on β-Catenin phosphorylation during p38MAPK and ERK1/2 dual inhibition in Huh7.5M cells. Cells were transfected with the respective plasmid for 48 hrs before the inhibitions.
