Figure 7

Analysis of cellular activities. (A) Live/dead assay results of NIH3T3 cells under different heating conditions. (scale bar: 100 μm) (B) Cell proliferation tests via CCK-8 assay. (*p < 0.001, NS = no significant difference) (C) F-actin (red)/DAPI (blue) images indicated cell spreading on day 7. (scale bar: 100 μm) (D) Analysis of the ratio of the stained area under different heating conditions.