Figure 4
From: Periostin-binding DNA aptamer treatment attenuates renal fibrosis under diabetic conditions
Changes in periostin, fibronectin, and type I collagen expression following periostin-binding DNA aptamer (PA) administration in inner medullary collecting duct (IMCD) cells treated with or without transforming growth factor-β1 (TGF-β1). (A) Periostin, fibronectin, and type I collagen mRNA levels (n = 6). Treatment with PA (100 pmol/ml) significantly ameliorated TGF-β1-induced (10 ng/ml) periostin, fibronectin, and type I collagen mRNA expression. (B) Representative western blotting analysis of periostin, fibronectin, and type I collagen protein levels (a representative of four blots). The increases in the protein levels of periostin, fibronectin, and type I collagen in TGF-β1-stimulated IMCD cells were significantly attenuated by the PA. Uncropped scans are presented in Supplementary Figure 4. (C) Cell viability in IMCD cells with or without treatment of PA and TGF-β1. Cell viability was not affected by PA treatment. However, cell viability was significantly decreased in TGF-β1-stimulated IMCD cells. C, control; PA, periostin-binding DNA aptamer. *P < 0.05 vs. C; **P < 0.05 vs. TGF-β1.
