Figure 6
FPS-ZM1 inhibited 6-OHDA-induced neuronal death. Tissues were prepared for immunofluorescence and western blotting 15 days after 6-OHDA administration. (a) Representative immunofluorescence images of SN immunostained for TH, NeuN, and DAPI (n = 10 per group). The ipsilateral sides are shown. The microscopy images were taken with 400 μm of magnification and the squares represents the approximation to 100 μm. (b) Representative western blots and quantification of NeuN (n = 6 per group) and c) TH (n = 6 per group). Each color in the graph represents a group and a brain location: gray - control/ipsilateral side; blue - FPS-ZM1/ipsilateral side; red - 6-OHDA/ipsilateral side; dark red - 6-OHDA/contralateral side; green - FPS-ZM1 + 6-OHDA/ipsilateral side; dark green - FPS-ZM1 + 6-OHDA/contralateral side. Values represent mean ± SD. One-way analysis of variance and Bonferroni Multiple Comparison post-hoc test were applied to all data. p values are embedded in the figure.
