Figure 1
YY1 facilitates the expression of MMP-14 in gastric cancer cells. (a) Scheme of potential binding sites of YY1 and miR-584-3p within the MMP-14 promoter, locating at bases −225/−219 and −167/−150 relative to TSS. (b) Western blot showing the expression of YY1 and MMP-14 in normal gastric epithelial GES-1 cells and gastric cancer cell lines SGC-7901, AGS, MKN-45, and MKN-28. (c and d) Western blot indicating the expression of YY1 and MMP-14 in SGC-7901, AGS and MKN-45 cells stably transfected with empty vector (mock), YY1, scramble shRNA (sh-Scb) or two YY1 shRNAs (sh-YY1 #1 and sh-YY1 #2). (e and f) Real-time quantitative RT-PCR showing the transcript levels of YY1 and MMP-14 in gastric cancer cells stably transfected with mock, YY1, sh-Scb or sh-YY1 (mean ± SD, n = 5). (g and h) Nuclear run-on assay indicating the nascent MMP-14 transcript levels in gastric cancer cells stably transfected with mock, YY1, sh-Scb, or sh-YY1 (mean ± SD, n = 4). *P < 0.01 vs. GES-1, mock, or sh-Scb.
