Figure 5
miR-584-3p attenuates the YY1-facilitated growth, metastasis, and angiogenesis of gastric cancer cells in vivo. (a) Tumor growth curve of SGC-7901 cells (1 × 106) stably transfected with empty vector (mock) or miR-584-3p precursor, and those co-transfected with YY1 in athymic nude mice (n = 5 for each group), after hypodermic injection for 4 weeks. (b) Representative images (top), weight (bottom), and miR-584-3p levels (bottom) of xenograft tumors formed by hypodermic injection of SGC-7901 cells stably transfected with mock or miR-584-3p precursor, and those co-transfected with YY1 (mean ± SD, n = 5). (c) Western blot showing the expression of YY1, MMP-14, VEGF165, and MMP-2 within tumors formed by hypodermic injection of SGC-7901 cells stably transfected with mock or miR-584-3p precursor, and those co-transfected with YY1 (mean ± SD, n = 5). (d) Double immunohistochemical staining (top) and quantification (bottom) of Ki-67 (red, arrowheads)/CD31 (brown) expression and necrosis area within tumors formed by hypodermic injection of SGC-7901 cells stably transfected with mock or miR-584-3p precursor, and those co-transfected with YY1 (mean ± SD, n = 5). Scale bars: 100 μm. (e) Representation (left, arrowheads) and quantification (middle) of lung metastasis and Kaplan–Meier survival plots (right) of nude mice with injection of SGC-7901 cells (0.4 × 106) stably transfected with mock or miR-584-3p precursor, and those co-transfected with YY1 via the tail vein (n = 5 for each group). Scale bars: 100 μm. *P < 0.001 vs. mock.
