Figure 4
From: Small molecules enhance CRISPR/Cas9-mediated homology-directed genome editing in primary cells
Effects of small molecules on HDR efficiency. (A) Schematic diagram of the eGFP reporter plasmid. (B) Detection of HDR efficiency in porcine fetal fibroblasts transfected the eGFP reporter by flow cytometry. (C) Summary of HDR efficiency in porcine fetal fibroblasts treated with different concentrations of the three small molecules. DMSO-treated reporter transfected cells were used as control. pEGFP-N1 transfected cells were used to set a gate for analyzing eGFP-positive cells. Values are shown as mean ± SEM. An asterisk represents P < 0.01, and double asterisks represent P < 0.05 versus control.
