Figure 3 | Scientific Reports

Figure 3

From: An expandable embryonic stem cell-derived Purkinje neuron progenitor population that exhibits in vivo maturation in the adult mouse cerebellum

Figure 3

In vitro replication of cerebellar anlage at the expansion stage. ES cell-derived cerebellar NPCs can be expanded in NPC medium. (a) Expression of nestin (labeling neurons plus astrocytes) and β-III-tubulin (only neurons) in NPCs from passages (p) 2–8. Scale bar indicates 20 μm. (b) Quantification of the percentage of nestin-positive or β-III-tubulin-positive NPCs of the total number of cells as indicated by DAPI-staining for passage 2–8 (averaged across 10 images of three experimental runs for each passage). Note that the percentage of NPCs drops after passage 6. Error bars represent SEM. Asterisks indicate significance levels comparing the average of a passage between all passages via one-way ANOVA followed by Tukey’s with statistical significance set at P ≤ 0.01: *P < 0.01, **P < 0.001, ***P < 0.0001.

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