Figure 7

Integration into the intact molecular layer and formation of synaptic connections with afferents. (a) Thirty days after transplantation into the adult (10-month old) wild type cerebella some of donor GFP-positive cells (arrows) enter the intact molecular layer of lobule simplex through the pial surface. (a,b) Donor cells differentiating into Purkinje cells (d–f) adopt the PN progenitor behavior. Once the cortex is entered, they migrate along the host Bergman glia stopping at different depths of the host molecular layer. (b,d) Distribution of VGLUT2 into the host molecular layer. (e) Even the displaced, donor GFP-positive, calbindin-positive cells grafted into the molecular layer receive synaptic inputs from host climbing fibers as shown by the presence of the VGLUT2-positive synaptic boutons on their dendrites. Scale bars: 50 μm (a,b); 20 μm (c,d); and 10 μm (e). *All micrographs represent coronal sections.