Figure 8

Adult (10-month old) wild type cerebella allow the synaptic integration of GFP-positive PNs in the host’s cerebellar network thirty days after transplantation to a limited extent. (a) Some of the donor GFP-positive cells enter the cortex in the vermis through the pial surface and settle in the host molecular layer where synaptic VGLUT2-positive boutons are located on the (b) proximal part of their dendrites. Arrow points to the axon of a displaced donor Purkinje cell, which is extending on the opposite side of the extensive dentritic tree. (c,d) The dendritic tree of a GFP-positive PN is enveloped by VGLUT2 positive boutons on its intermediary and distal parts. (e) VGLUT1-positive vesicles surround grafted GFP-positive PNs and (f) its distal dendritic tree. Axon is indicated by arrow. Scale bars: 10 μm (a,c,f); 5 μm (b,d); and 20 μm (e). *All micrographs represent coronal sections.