Figure 9

NPC injections in L7cre-ERCC1 KO mice matured into PNs. (a,b) NPCs expressing actin-GFP were injected into lobules IV-V of an 8-month old mouse cerebellum and displayed maturation as shown by calbindin expression and morphological features. (c) Some GFP-positive PNs migrated to the approximate location where the Purkinje cell layer used to be before degeneration. All images were taken 25 days after grafting (n = 3 mice). Scale bars: 100 μm (a); and 40 µm (b,c). ML: Molecular layer; PCL: Purkinje cell layer; GCL: Granule cell layer. *All micrographs represent coronal sections.