Figure 2 | Scientific Reports

Figure 2

From: Mutation of the Slt2 ortholog from Cryphonectria parasitica results in abnormal cell wall integrity and sectorization with impaired pathogenicity

Figure 2

Characteristics of the apical hyphal growth units in the CpSlt2-null mutant strain. (A) Microscopic observation of apical hyphae of the CpSlt2-null mutant strain. Strains, indicated on the panels, are wild-type EP155/2, TdSLT2-69, a sectored progeny of TdSLT2-69 (TdSLT2-69-S1), a complemented strain of TdSLT2-69 (TcSLT2-69), and the CpBck1-null mutant (TdBCK1) strain. Note that arrowheads in the enlarged inlet represent examples of the apical hyphal growth unit and the sectored strains are moderately more geniculate or inflective as indicated by the arrow in the enlarged inlet at the bottom right. Black bars represent 50 μm. (B) Apical growth units were measured by the hyphal length from the apex to the third branching point. At least 40 individual hyphae were observed for each strain and each experiment was repeated three times. Data followed by the same letters above the bars are not significantly different between treatments, according to the Tukey HSD test at p = 0.01.

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