Figure 2
From: Light-patterning of synthetic tissues with single droplet resolution
Single-droplet activation in a droplet pair demonstrated by electrical recording. (a) Schematic representing the light-activated expression of the pore-forming protein αHL in a single droplet of a droplet pair. (b) Schematic showing the use of two electrode-containing droplets to interrogate the droplets in the pair. In position 1, ions can pass through the ‘ON’ droplet of the pair (droplet encircled in green). When one of the electrode-containing droplets is moved to position 2, the ion flux can no longer move through the system, as the ‘OFF’ droplet (droplet encircled in red) of the pair does not contain αHL. (c) Image of a droplet network with electrode droplets in position 1. (d) Electrical recording performed in position 1. (e) Image of a droplet network with electrode droplets in position 2. (f) Electrical recording performed in position 2. (g) Voltage protocol used for ‘d’ and ‘f’. Scale bars in ‘c’ and ‘e’, 200 μm.
