Figure 4
From: Light-patterning of synthetic tissues with single droplet resolution
Light-patterning different levels of protein expression in multiple single pL-sized droplets within a synthetic tissue. (a) Droplets within a printed synthetic tissue, containing LA-DNA encoding mVenus, were light-activated for different durations, producing different levels of expressed protein in each droplet. The central droplet was activated for 60 s, and the surrounding six droplets were illuminated for 30 s and 15 s, in an alternating pattern. The droplets surrounding the seven activated droplets were not illuminated. Scale bar, 75 μm. (b) Three fluorescence intensity line profiles through the hexagon shape in ‘a’. (c) Plot showing the mean and standard deviation of the fluorescence intensity across each droplet type, an indicator of the level of expression, for a particular droplet type against the corresponding illumination time.
