Figure 5
From: Light-patterning of synthetic tissues with single droplet resolution

Reversible activation of the fluorescent protein rsTagRFP in single nL-sized droplets within a droplet pair and within droplet networks. (a) Schematic representing the activation and deactivation of the reversible fluorescent protein rsTagRFP in a droplet pair. (b) The fluorescence of single droplets in a droplet pair can be turned OFF with light, the whole system reactivated, then a different droplet turned OFF. (c) Fluorescence intensity line profiles from ‘b’. (d) Turning OFF the fluorescence in single droplets of a three-droplet network. (e) Fluorescence intensity line profiles from ‘d’. (f) Turning OFF the fluorescence in single droplets of a seven-droplet network. Scale bars, 250 μm.