Figure 5
In vivo pHi mapping of HeLa-SypHer2 tumors after treatment with cisplatin. (A) Fluorescence images with excitation at 430 nm and 500 nm (detection at 540 nm); (B) images of SypHer2 ratio (I500/I430) from three untreated (upper row) and three treated (lower row) tumors in vivo; (C) quantitative analysis of the SypHer2 ratio in the tumors in vivo, mean ± SD, n = 3 tumors; (D) SypHer2 ratio from freshly frozen tissue sections. Bar is 100 µm. The cryosections from the HeLa-SypHer2 tumors correspond to the histological slices shown in Fig. 4B. Images were acquired on the 35th day of tumor growth (3 days after the final cisplatin dose). *Statistically significant difference from the untreated control, p ≤ 0.05.
