Figure 6
In vivo two-photon FLIM of NAD(P)H in HeLa tumors in response to cisplatin. (A) Fluorescence lifetimes of NAD(P)H (short t1 and long t2 components), relative amplitude of the short component (free NAD(P)H, a1, %), and overlapping of two-photon excited autofluorescence (AF, green, excitation at 750 nm, detection 410–660 nm) and SHG from collagen (red, excitation at 750 nm, detection - 373–387 nm). (B) Relative amplitude a1% of free NAD(P)H calculated from the in vivo FLIM images. *Statistically significant difference from the untreated control, p ≤ 0.05, mean ± SD, n = 3 tumors (the calculations were made for 50–75 cells in each tumor). FLIM was performed on the 35th day of tumor growth (3 days after the final cisplatin dose). For FLIM of NAD(P)H: ex. 750 nm, reg. 410–660 nm, image collection time was 10 seconds. Bar is 100 µm (applicable to all images).
