Figure 2
Effect of MeHg exposure and ER stress preconditioning on mTORC1 signaling. (A) Effect of ER stress preconditioning on mTORC1 signaling following exposure to 0.5 μM MeHg. The histogram depicts Mtor mRNA normalized to Actb mRNA analyzed by RT-qPCR. Values shown are the means ± SE of 4 separate experiments. ***Significantly different from TPG-untreated cells by a one-way ANOVA (here p < 0.001). ##, ###Significantly different from TPG-treated and MeHg-untreated cells by a one-way Welch’s t-test (##p < 0.01, ###p < 0.001). §Significantly different from TPG- and MeHg-untreated cells by a one-way Welch’s t-test (here p < 0.05). (B) Western blotting analysis for phospho-4EBP1 (p-4EBP1). Total cell lysates prepared at the times indicated were analyzed using the indicated antibody probes. Cropped blots are shown; all gels were run under the same experimental conditions.
